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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
26/11/2009 |
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Data da última atualização: |
15/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MEO, S. C.; FERREIRA, C. R.; PERECIN, F.; SARAIVA, N. Z.; TETZNER, T. A. D.; YAMAZAKI, W.; LEA, C. L. V.; MEIRELLES, F. V.; GARCIA, J. M. |
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Afiliação: |
SIMONE CRISTINA MEO NICIURA, CPPSE; CHRISTINA RAMIRES FERREIRA, USP; FELIPE PERECIN, USP; NAIARA ZOCCAL SARAIVA, UNESP; TATIANE ALMEIDA DRUMMOND TETZNER, UNESP; WALT YAMAZAKI, UNESP; CLÁUDIA LIMA VERDE LEA, USP; FLÁVIO VIEIRA MEIRELLES, USP; JOAQUIM MANSANO GARCIA, UNESP. |
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Título: |
Karyoplast exchange between strontium- and 6-DMAP-parthenogenetically activated zygotes of cattle. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Animal Reproduction Science, v. 116, n. 3-4, p. 381-385, dec. 2009. |
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DOI: |
https://doi.org/10.1016/j.anireprosci.2009.02.020 |
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Idioma: |
Inglês |
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Conteúdo: |
Ooplasmic factors drive nuclear organization after fertilization and are also important for re-programming in nuclear transfer procedures, in which artificial activation is essential for reconstructed embryos to progress in development. The present research evaluated the effect of pronuclear transfer (PT) between zygotes parthenogenetically activated with ionomycin followed by strontium (S) or 6-DMAP (D) on early embryonic development. PT was performed in the same zygote to obtain embryos in control groups (S-PT and D-PT) and between zygotes activated with S and D to achieve embryos with differentially activated cytoplasm (C) and nucleus (N) (SCDN and DCSN). PT procedure did not affect cleavage and blastocyst rates, respectively, in PT control groups compared to non-manipulated control (S-PT: 73.6% and 7.3% compared with S-Control: 77.9% and 7.8%; and D-PT: 73.3% and 31.7% compared with D-Control: 83.1% and 41.5%). Cleavage, eight-cell, and blastocyst rates, respectively, were similar between SCDN (76.5%, 36.4%, and 6.8%) and DCSN (69.5%, 25.0%, and 4.9%) embryos. Developmental rates in SCDN were similar to S-PT, but inferior to D-PT. Developmental arrest up to eight-cell stage was greater in SCDN and DCSN than in S-PT and D-PT. In conclusion, karyoplast exchange between parthenogenetic zygotes activated with strontium and 6-DMAP can lead to nuclear?cytoplasmic incompatibilities and affect embryonic development to the eight-cell and blastocyst stages. |
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Palavras-Chave: |
Artificial activation; Bovine; Embryonic development; Pronuclear transfer. |
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Thesaurus Nal: |
beef cattle. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02365naa a2200289 a 4500
001 1576277
005 2023-03-15
008 2009 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.anireprosci.2009.02.020$2DOI
100 1 $aMEO, S. C.
245 $aKaryoplast exchange between strontium- and 6-DMAP-parthenogenetically activated zygotes of cattle.$h[electronic resource]
260 $c2009
520 $aOoplasmic factors drive nuclear organization after fertilization and are also important for re-programming in nuclear transfer procedures, in which artificial activation is essential for reconstructed embryos to progress in development. The present research evaluated the effect of pronuclear transfer (PT) between zygotes parthenogenetically activated with ionomycin followed by strontium (S) or 6-DMAP (D) on early embryonic development. PT was performed in the same zygote to obtain embryos in control groups (S-PT and D-PT) and between zygotes activated with S and D to achieve embryos with differentially activated cytoplasm (C) and nucleus (N) (SCDN and DCSN). PT procedure did not affect cleavage and blastocyst rates, respectively, in PT control groups compared to non-manipulated control (S-PT: 73.6% and 7.3% compared with S-Control: 77.9% and 7.8%; and D-PT: 73.3% and 31.7% compared with D-Control: 83.1% and 41.5%). Cleavage, eight-cell, and blastocyst rates, respectively, were similar between SCDN (76.5%, 36.4%, and 6.8%) and DCSN (69.5%, 25.0%, and 4.9%) embryos. Developmental rates in SCDN were similar to S-PT, but inferior to D-PT. Developmental arrest up to eight-cell stage was greater in SCDN and DCSN than in S-PT and D-PT. In conclusion, karyoplast exchange between parthenogenetic zygotes activated with strontium and 6-DMAP can lead to nuclear?cytoplasmic incompatibilities and affect embryonic development to the eight-cell and blastocyst stages.
650 $abeef cattle
653 $aArtificial activation
653 $aBovine
653 $aEmbryonic development
653 $aPronuclear transfer
700 1 $aFERREIRA, C. R.
700 1 $aPERECIN, F.
700 1 $aSARAIVA, N. Z.
700 1 $aTETZNER, T. A. D.
700 1 $aYAMAZAKI, W.
700 1 $aLEA, C. L. V.
700 1 $aMEIRELLES, F. V.
700 1 $aGARCIA, J. M.
773 $tAnimal Reproduction Science$gv. 116, n. 3-4, p. 381-385, dec. 2009.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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Classificação |
Cutter |
Registro |
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Registro Completo
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
17/05/2018 |
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Data da última atualização: |
29/11/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 3 |
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Autoria: |
BRAZ, A. J. B. P.; FERREIRA, C. J. B.; SILVEIRA, P. M. da; SIMON, G. A.; ASSIS, R. L. de; BRAZ, G. B. P.; MEDEIROS, R. V. |
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Afiliação: |
ANTONIO JOAQUIM BRAGA PEREIRA BRAZ, UNIVERSIDADE DE RIO VERDE, Rio Verde-GO; CAMILA JORGE BERNABÉ FERREIRA, UNIVERSIDADE ESTADUAL DE MARINGÁ; PEDRO MARQUES DA SILVEIRA, CNPAF; GUSTAVO ANDRE SIMON, UNIVERSIDADE DE RIO VERDE, Rio Verde-GO; RENATO LARA DE ASSIS, INSTITUTO FEDERAL GOIANO, Iporá-GO; GUILHERME BRAGA PEREIRA BRAZ, UNIVERSIDADE DE RIO VERDE, Rio Verde-GO; RENATO VIEIRA MEDEIROS, mestrando UNIVERSIDADE DE RIO VERDE, Rio Verde-GO. |
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Título: |
Nitrogen fertilization in super-early cycle common bean using new sources of urea. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Científica, v. 46, n. 2, p. 180-186, 2018. |
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ISSN: |
1984-5529 |
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DOI: |
10.15361/1984-5529.2018v46n2p180-186 |
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Idioma: |
Inglês |
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Conteúdo: |
The efficiency of new fertilizers as a nitrogen source in super-early cycle common bean cultivars still needs to be better known. The objective was to quantify the effect of new sources and time of application of nitrogen on yield and yield components of a super-early cycle bean cultivar. The experiment was conducted in the city of Rio Verde, state of Goias, Brazil, using the experimental design of randomized blocks, in a 3x3+1 factorial scheme, with four replications. The first factor was constituted by nitrogen (N) sources: traditional urea, zeolite-associated urea, and urea with urease inhibitor, using the dose of 80 kg ha-1 N. The second factor was application stages: V2, V3, and V4, that is, when 50% of the plants had the first pair of unifoliolate leaves, the first trifoliolate leaf, and the third trifoliolate leaf fully expanded, respectively. Moreover, an additional treatment was used, without N application (control). The cultivar used was BRS FC104, of super-early cycle, from 60 to 65 days. The following were measured: relative chlorophyll index (RCI), plant height, first pod insertion height, number of pods per plant, number of grains per pod, mass of 100 grains and yield. There was no significant difference between sources and application stages regarding the number of pods per plant, number of grains per pod, plant height and first pod insertion height. The highest yields were obtained with the use of zeolite-associated urea, and the best application stage was V4. The use of urea with urease inhibitor did not provide an increase in grain yield compared to the control treatment. MenosThe efficiency of new fertilizers as a nitrogen source in super-early cycle common bean cultivars still needs to be better known. The objective was to quantify the effect of new sources and time of application of nitrogen on yield and yield components of a super-early cycle bean cultivar. The experiment was conducted in the city of Rio Verde, state of Goias, Brazil, using the experimental design of randomized blocks, in a 3x3+1 factorial scheme, with four replications. The first factor was constituted by nitrogen (N) sources: traditional urea, zeolite-associated urea, and urea with urease inhibitor, using the dose of 80 kg ha-1 N. The second factor was application stages: V2, V3, and V4, that is, when 50% of the plants had the first pair of unifoliolate leaves, the first trifoliolate leaf, and the third trifoliolate leaf fully expanded, respectively. Moreover, an additional treatment was used, without N application (control). The cultivar used was BRS FC104, of super-early cycle, from 60 to 65 days. The following were measured: relative chlorophyll index (RCI), plant height, first pod insertion height, number of pods per plant, number of grains per pod, mass of 100 grains and yield. There was no significant difference between sources and application stages regarding the number of pods per plant, number of grains per pod, plant height and first pod insertion height. The highest yields were obtained with the use of zeolite-associated urea, and the best application stage was V4... Mostrar Tudo |
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Thesagro: |
Adubação; Feijão; Fertilizante Nitrogenado; Uréase. |
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Thesaurus NAL: |
Beans; Fertilizer application; Nitrogen fertilizers; Urease inhibitors; Zeolites. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/177134/1/CNPAF-2018-cientifica.pdf
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Marc: |
LEADER 02543naa a2200325 a 4500
001 2091565
005 2018-11-29
008 2018 bl uuuu u00u1 u #d
022 $a1984-5529
024 7 $a10.15361/1984-5529.2018v46n2p180-186$2DOI
100 1 $aBRAZ, A. J. B. P.
245 $aNitrogen fertilization in super-early cycle common bean using new sources of urea.$h[electronic resource]
260 $c2018
520 $aThe efficiency of new fertilizers as a nitrogen source in super-early cycle common bean cultivars still needs to be better known. The objective was to quantify the effect of new sources and time of application of nitrogen on yield and yield components of a super-early cycle bean cultivar. The experiment was conducted in the city of Rio Verde, state of Goias, Brazil, using the experimental design of randomized blocks, in a 3x3+1 factorial scheme, with four replications. The first factor was constituted by nitrogen (N) sources: traditional urea, zeolite-associated urea, and urea with urease inhibitor, using the dose of 80 kg ha-1 N. The second factor was application stages: V2, V3, and V4, that is, when 50% of the plants had the first pair of unifoliolate leaves, the first trifoliolate leaf, and the third trifoliolate leaf fully expanded, respectively. Moreover, an additional treatment was used, without N application (control). The cultivar used was BRS FC104, of super-early cycle, from 60 to 65 days. The following were measured: relative chlorophyll index (RCI), plant height, first pod insertion height, number of pods per plant, number of grains per pod, mass of 100 grains and yield. There was no significant difference between sources and application stages regarding the number of pods per plant, number of grains per pod, plant height and first pod insertion height. The highest yields were obtained with the use of zeolite-associated urea, and the best application stage was V4. The use of urea with urease inhibitor did not provide an increase in grain yield compared to the control treatment.
650 $aBeans
650 $aFertilizer application
650 $aNitrogen fertilizers
650 $aUrease inhibitors
650 $aZeolites
650 $aAdubação
650 $aFeijão
650 $aFertilizante Nitrogenado
650 $aUréase
700 1 $aFERREIRA, C. J. B.
700 1 $aSILVEIRA, P. M. da
700 1 $aSIMON, G. A.
700 1 $aASSIS, R. L. de
700 1 $aBRAZ, G. B. P.
700 1 $aMEDEIROS, R. V.
773 $tCientífica$gv. 46, n. 2, p. 180-186, 2018.
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Embrapa Arroz e Feijão (CNPAF) |
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